Fig. 3.

Aggression-related decrease in brain metabolism as a function of heredity, alarm pheromone exposure, and age. Results of assays of enzyme activity for Complex I (NADH dehydrogenase), IV (cytochrome c oxidase), and V (ATP synthase) performed on mitochondrial preparations from bee brains. n = 4 biological replicates for each of the six groups labeled on the x axes of the three graphs; two pools of five brains per colony per behavioral group from two colonies. n = 3 technical replicates per sample. Statistical analysis: 1-way ANOVA with Tukey HSD posthoc. EHB, EHB bees in EHB colonies; AHB, AHB bees in AHB colonies. See SI Text for methods. Means ± SD are shown.