Fig. 1.

Dynamic association of miRNAs with AIN-2-miRISCs during development. (A) Heat map illustration of log2-transformed miRNA concentrations measured by pyrosequencing in 18 AIN-2::GFP IP and five total RNA samples from worms at five indicated developmental stages. Each row represents a miRNA and each column represents an individual sample. Only the 86 detected miRNAs were included. Different columns for the same stage are different replicates for that stage. The heat map and tree structure were generated by the self-organization map approach followed by complete linkage hierarchical clustering using the cluster software. The miRNAs can be grouped into four major clusters: all-stage miRNAs (1), early-stage miRNAs (2), late-stage miRNAs (3) and miRNAs with sporadic low level expressions (4). (B) The expression pattern of lin-4 and let-7 family miRNAs determined by pyrosequencing. The results were taken directly from the total RNA samples displayed in A.