Fig. 3.

Reporter analysis of dynamics of miRNA regulation on the expression of four genes during development. (A) Cartoon illustration of the dual-color reporter analysis strategy. As the control for transgene expression, rpl-28 driving his-24::mCherry:let-858 3′UTR was co-expressed with the GFP reporters in each animal from the same extra-chromosomal array. (B-F) Fluorescence images of living animals expressing GFP reporters of four indicated 3′UTRs. The stage of each image is indicated. All GFP reporters contain a 4× SV40 nuclear localization signal (NLS) and were driven by the ubiquitous rpl-28 promoter. The mCherry images were taken immediately after the GFP images of the same animals using exactly the same scope settings.