Table 2.
Analysis of 3′UTR reporter expression using transgenic animals
| 3′UTR (line number) | Reference tissue | GFP intensity | Egg | L1,L2 | L3,L4 |
|---|---|---|---|---|---|
| let-858 (A) | All | ++ | 97.0%±3.0% | 97.2%±2.8% | 100%±0.0% |
| + | 3.0%±3.0% | 2.8%±2.8% | 0.0%±0.0% | ||
| − | 0.0%±0.0% | 0.0%±0.0% | 0.0%±0.0% | ||
| n | 46 | 56 | 77 | ||
| let-858 (B) | All | ++ | 73.2%±1.0% | 92.3%±3.9% | 96.2%±0.3% |
| + | 26.8%±1.0% | 7.7%±3.9% | 3.8%±0.3% | ||
| − | 0.0%±0.0% | 0.0%±0.0% | 0.0%±0.0% | ||
| n | 136 | 67 | 80 | ||
| hbl-1 (A) | All | ++ | 0.0%±0.0% | 0.0%±0.0% | 0.0%±0.0% |
| + | 94.1%±3.4% | 72.7%±3.9% | 5.8%±3.3% | ||
| − | 5.9%±3.4% | 27.3%±3.9% | 94.2%±3.3% | ||
| n | 46 | 70 | 88 | ||
| hbl-1 (B) | All | ++ | 0.0%±0.0% | 0.0%±0.0% | 0.0%±0.0% |
| + | 64.5%±1.5% | 74.2%±0.8% | 6.3%±6.3% | ||
| − | 35.5%±1.5% | 25.8%±0.8% | 93.8%±6.3% | ||
| n | 79 | 62 | 80 | ||
| egl-1 (A) | Intestine | ++ | 0.0%±0.0% | 26.6%±3.1% | |
| + | 21.3%±1.5% | 71.6%±2.2% | |||
| − | 78.7%±1.5% | 1.9%±1.9% | |||
| n | 99 | 89 | |||
| egl-1 (B) | Intestine | ++ | 1.7%±1.7% | 3.1%±1.5% | |
| + | 25.6%±0.6% | 93.0%±2.4% | |||
| − | 72.7%±1.5% | 3.9%±3.9% | |||
| n | 70 | 85 | |||
| sqv-3 (A) | All | ++ | 1.7%±1.7% | 6.0%±1.2% | |
| + | 76.1%±21.4% | 94.0%±1.2% | |||
| − | 22.2%±22.2% | 0.0%±0.0% | |||
| n | 67 | 66 | |||
| sqv-3 (B) | All | ++ | 2.5%±1.4% | 10.8%±0.9% | |
| + | 81.7%±15.9% | 89.2%±0.9% | |||
| − | 15.8%±15.8% | 0.0%±0.0% | |||
| n | 70 | 74 | |||
| twk-12 (A) | All | ++ | 0.0%±0.0% | 0.0%±0.0% | |
| + | 2.8%±2.8% | 1.1%±1.1% | |||
| − | 97.2%±2.8% | 98.9%±1.1% | |||
| n | 55 | 79 | |||
| twk-12 (B) | All | ++ | 0.0%±0.0% | 0.0%±0.0% | |
| + | 1.8%±1.8% | 1.2%±1.2% | |||
| − | 98.2%±1.8% | 98.8%±1.2% | |||
| n | 64 | 62 | |||
| egl-1 3′UTR+mir-35 (A) | Intestine | ++ | 0.0%±0.0% | ||
| + | 10.4%±3.1% | ||||
| − | 89.6%±3.1% | ||||
| n | 76 | ||||
| egl-1 3′UTR+mir-35 (B) | Intestine | ++ | 0.0%±0.0% | ||
| + | 18.1%±0.8% | ||||
| − | 81.9%±0.8% | ||||
| n | 120 |
Each of the rpl-28 promoter:4NLS::gfp::candidate 3′UTRs constructs was co-injected with the reference rpl-28 promoter:his-24::mCherry::let-858 3′UTRs construct to create transgenic animals. Two independent transgenic lines were analyzed for each candidate 3′UTR (line A and line B). The GFP expression level was measured by comparing the fluorescence intensity of GFP with that of mCherry. The percentage of animals with each GFP expression level in each transgenic line at indicated development stages was counted and listed in the table.
++, GFP fluorescence is stronger than or comparable with the mCherry fluorescence.
+, GFP fluorescence is visible but much weaker than the mCherry fluorescence.
−, GFP fluorescence is mostly invisible despite the presence of strong mCherry fluorescence.
n indicates the total number of counted animals.
Reference tissue indicates the tissue that the counting was based on.