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. 2009 Aug 12;136(18):3067–3075. doi: 10.1242/dev.036426

Fig. 2.

Fig. 2.

P-constructs used in these experiments. (A) Constructs with the en promoter. P[en3R] {P[en1] from Kassis et al. (Kassis et al., 1992)} and P[en3] (Devido et al., 2008) contain en sequences (green) extending from -2.407 kb through to the start site of en transcription (indicated by the arrow) and 188 bp of the en untranslated leader fused to Adh-lacZ (blue). P[en3Δboth] contains en sequences from -395 bp through to +188 bp (Devido et al., 2008). L, loxP site; F, FRT site; P, P-element ends. (B) Constructs with the heat shock promoter (HSP). The pUZ vector is shown along with the insertion site for the en fragments (green) (Lyko et al., 1997). The locations of two Polycomb response elements (PREs) are shown as green boxes (DeVido et al., 2008; Kassis, 1994). The extent of the en fragment in each construct is shown by the green line.