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A promoter-tethering element is contained within PRE2. The name of the line is on top, with a wing disc (left) and a leg disc (right) shown underneath each line. Discs were fixed with formaldehyde and stained for varying times: P[en3R]-210bp and P[en3Δboth]-en(-6kb), 45 minutes; P[enHSP2]-250bp, 2 hours; P[enHSP]-250bp, 29 hours.
