Table 1.
Insertion site of transgenes studied
| Construct | Line | Gene* | Location† |
|---|---|---|---|
| P[en3R] | +130kb | sprt | 7,285,340 |
| P[enHSP1] | +75kb | E(Pc) | 7,340,264 |
| P[en3R] | +75kb | E(Pc) | 7,340,298 |
| P[en3R] | +75kb-2 | E(Pc) | 7,340,671 |
| P[en3R] | +52kb | inv | 7,363,188 |
| P[en3R] | +52kb-2 | inv | 7,363,197 |
| P[en3R] | +52kb-3 | inv | 7,363,221 |
| enGAL4 | (+42kb) | inv | 7,373,480 |
| P[enHSP1] | +28kb | inv | 7,387,233 |
| P[enHSP1] | +28kb+19bp | inv | 7,387,340 |
| P[enHSP1] | +19bp | en | 7,415,369 |
| P[enHSP1] | +28kb+19bp | en | 7,415,369 |
| P[en3R] | −204bp | en | 7,415,592 |
| P[en3R] | −210bp | en | 7,415,598 |
| P[enHSP3] | −250bp | en | 7,415,638 |
| P[enHSP2] | −250bp | en | 7,415,638 |
| P[en3R] | −412bp | en | 7,415,800 |
| P[enHSP2] | −1kb | en | 7,416,417 |
| P[en3] | en (−6kb) | en | 7,421,628 |
| P[en3Δboth] | en (−6kb) | en | 7,421,628 |
| P[en3] | tou (−66kb) | tou | 7,481,428 |
| P[enHSP1] | −67kb | tou | 7,482,731 |
| P[enHSP1] | −67kb-2 | tou | 7,482,786 |
| P[lacW] | tou1 (−73kb) | tou | 7,488,577 |
| P[enHSP1] | −74kb | tou | 7,489,278 |
| P[en3R] | −77kb | tou | 7,492,176 |
| P[en3R] | −77kb-1 | tou | 7,492,223 |
| P[en3R] | −77kb-2 | tou | 7,492,778 |
| P[en3R] | −86kb | tou | 7,501,843 |
| P[en3R] | −87kb | tou | 7,502,613 |
| P[en3R] | −163kb | wal | 7,578,342 |
Line names indicate the number of kilobases or bases upstream (negative numbers) or downstream (positive numbers) of the major en transcription start site (at 7,415,388) (Soeller et al., 1988). A few lines were named previously. For these, the number of kilobases from the en transcription start site is shown in parentheses.
*
Gene inserted in or next to.
†
Nucleotide insertion site (genome version R5.1, FlyBase).