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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Fertil Steril. 2008 Aug 9;91(5 Suppl):2051–2060. doi: 10.1016/j.fertnstert.2008.05.069

TABLE 2.

Proportions of nuclear stages in groups of denuded oocytes (DOs) and cumulus-oocytes complexes (COCs) after 28 h of culture with or without FSH/LH, in the presence or absence of 1-heptanol, and either separately or in co-culture.

FSH/LH 1-heptanol n % GV* % GVBD* % MI* % MII*
DOs cultured separately 69 94.2a 0.0a 0.0a 0.0a
DOs cultured separately + 77 93.5a 0.0a 0.0a 0.0a
DOs cultured separately + 78 0.0b 32.1b 41.0b 21.8b
DOs cultured separately + + 80 0.0b 30.0b 37.5b 27.5b
COCs cultured separately 77 0.0b 39.0b 58.4c 0.0a
COCs cultured separately + 74 95.9a 0.0a 0.0a 0.0a
COCs cultured separately + 70 0.0b 2.9ac 14.3d 78.6c
COCs cultured separately + + 80 0.0b 27.5b 42.5b 25.0b
DOs co-cultured with COCs 71 97.2a 0.0a 0.0a 0.0a
COCs co-cultured with DOs 80 0.0b 37.5b 60.0c 0.0a
DOs co-cultured with COCs + 73 98.6a 0.0a 0.0a 0.0a
COCs co-cultured with DOs + 75 96.0a 0.0a 0.0a 0.0a
DOs co-cultured with COCs + 85 0.0b 5.9c 11.8d 78.8c
COCs co-cultured with DOs + 83 0.0b 6.0c 15.7d 74.7c
DOs co-cultured with COCs + + 81 0.0b 30.9b 38.3b 29.6b
COCs co-cultured with DOs + + 80 0.0b 31.3b 40.0b 23.8b
*

Germinal Vesicle (GV), germinal vesicle breakdown (GVBD), metaphase I (MI), metaphase II (MII).

Within the same column, values with different superscripts differ (P < 0.05; Chi-square test).