Fig. 4.

Hydroquinone inhibited TrHBMEC tube formation in matrigel. Hydroquinone did not induce obvious morphological change and cell death when TrHBMECs were seeded on gelatin-coated plates (A and B). However, hydroquinone inhibited TrHBMEC tube formation in a dose-dependent manner when cells were seeded on matrigel-coated plates; 10 μM hydroquinone almost completely inhibited TrHBMEC tube formation (C-E). Arrows indicate the tube structures. Data represent similar results from three independent experiments. Total tube length per field was measured by image processing and analysis software. ^*, p < 0.05 is considered significant compared with untreated control. ^**, p < 0.05 is considered significant compared with untreated control and 5 μM hydroquinone treatment (F).