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. 2009 Jun 12;76(3):579–587. doi: 10.1124/mol.109.057323

Fig. 7.

Fig. 7.

Overexpression of NQO1 in TrHBMECs protects cells from hydroquinone-induced up-regulation of ChM-I and inhibition of tube formation. TrHBMECs were first transfected with either control vector or NQO1 overexpression vector for 18 to 24 h; blot (A) demonstrated a successful overexpression of NQO1 in TrHBMECs. Cells were then subjected to hydroquinone treatment or the tube-formation assay in the presence or absence of 10 μM hydroquinone. ChM-I precursor protein levels after hydroquinone challenge were examined in TrHBMEC sonicates by immunoblot analysis. Overexpression of NQO1 inhibited hydroquinone-induced up-regulation of ChM-I (B). β-Actin was included as a loading control. Hydroquinone (10 μM) inhibited tube formation in TrHBMECs transfected with control vector (C, subpanels A and C) but the inhibition was abrogated in TrHBMEC transfected with NQO1 overexpression vector (C, subpanels B and D). Arrows indicate the tube structures. Data represent similar results from three independent experiments. Total tube length per field was measured by image processing and analysis software. *, p < 0.05 (D).