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. 2009 Jul 15;136(16):2725–2733. doi: 10.1242/dev.038596

Fig. 3.

Fig. 3.

Specificity of the anti-KLC-2 and anti-UNC-116 antibodies. (A) A western blot (developed on film) with anti-UNC-116 antibodies. A single band of ∼97 kDa is detected in immunoblots of mixed-stage wild-type worms (lane 1) as well as unc-116(rh24sb79) (lane 2) and unc-116(e2310) (lane 3) animals. A single band of ∼113 kDa in immunoblots of mixed-stage unc-116(f122); UNC-116::GFP animals corresponds to the expected size of UNC-116::GFP (lane 4). (B) A western blot (imaged by digital camera) with anti-KLC-2 antibodies. Bands were detected of ∼66 kDa in wild-type embryos (lane 1) and 97 kDa in klc-2(km28); KLC-2::GFP embryos, corresponding to KLC-2::GFP (lane 2). (C,D) Wide-field fluorescence images of wild-type embryos stained with the anti-KLC-2 or UNC-116 antibodies. (C) KLC-2 expression is low in early embryos, such as in the 4-cell stage embryo shown (arrowhead). Expression increases greatly in the pre-elongation stage (arrow). (D) UNC-116 expression in early embryos is relatively low and diffuse, such as in the 8-cell embryo shown (arrowhead). Expression is much higher and more organized in the pre-elongation stage (arrow). Scale bar: 10 μm.