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. 2009 Jul 15;136(16):2725–2733. doi: 10.1242/dev.038596

Fig. 6.

Fig. 6.

The KLC-2::KASH construct can partially bypass the requirement of UNC-83 for hyp7 nuclear migration. (A) The average number of hyp7 nuclei counted in the dorsal cord of unc-83(e1408) null animals (n=71) and two lines of unc-83(e1408); ycEx117,118[pSL440(KLC-2::myc::KASH)] (KLC-2::myc::KASH i, ii) (n=20). Error bars indicate s.e. (B-E) Single-slice confocal images of pre-elongation embryos. (B) An unc-83(e1408); ycEx118[pSL440(KLC-2::myc::KASH)] (KLC-2::myc::KASH ii) embryo stained with the anti-KLC-2 antibody. (C) The same embryo and focal plane as in B, stained with an anti-myc antibody. (D) An unc-83(e1408); ycEx118[pSL440(KLC-2::myc::KASH)] (KLC-2::myc::KASH ii) embryo stained with the anti-UNC-116 antibody. (E) The same embryo and focal plane as in D, stained with an anti-myc antibody. Dorsal views with anterior on the left. Scale bar: 10 μm.