Overexpression of CEH-51 promotes specification of MS-derived cell
types. (A,F) A small number of cells expressing
ceh-22::GFP are restored to skn-1(RNAi) embryos by
hs-ceh-51. (B,G) Expression of the pharynx muscle gene
myo-2 is largely absent in both skn-1(RNAi) and
skn-1(RNAi); hs-ceh-51 embryos. The inset in B shows
wild-type expression of myo-2. (C,H) Many
hs-ceh-51 embryos display unc-120::GFP-expressing cells in a
skn-1(RNAi); pal-1(RNAi) background, which depletes embryos of nearly
all body muscles (Hunter and Kenyon,
1996). (D,I) In a skn-1(zu67); pal-1(RNAi)
background, heat shock of ceh-51 causes the generation of many cells
expressing the muscle myosin gene myo-3. One hundred percent
(n=79) of heat shocked non-transgenic embryos resembled those shown
in D, whereas 53% (n=53) of heat shocked transgenics resembled those
shown in I. (E,J) hs-ceh-51 embryos accumulate ectopic
coelomocytes. (K-M) Bar charts summarizing the hs-ceh-51
data.