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. 2009 Jul 15;136(16):2735–2746. doi: 10.1242/dev.038307

Fig. 5.

Fig. 5.

Overexpression of CEH-51 promotes specification of MS-derived cell types. (A,F) A small number of cells expressing ceh-22::GFP are restored to skn-1(RNAi) embryos by hs-ceh-51. (B,G) Expression of the pharynx muscle gene myo-2 is largely absent in both skn-1(RNAi) and skn-1(RNAi); hs-ceh-51 embryos. The inset in B shows wild-type expression of myo-2. (C,H) Many hs-ceh-51 embryos display unc-120::GFP-expressing cells in a skn-1(RNAi); pal-1(RNAi) background, which depletes embryos of nearly all body muscles (Hunter and Kenyon, 1996). (D,I) In a skn-1(zu67); pal-1(RNAi) background, heat shock of ceh-51 causes the generation of many cells expressing the muscle myosin gene myo-3. One hundred percent (n=79) of heat shocked non-transgenic embryos resembled those shown in D, whereas 53% (n=53) of heat shocked transgenics resembled those shown in I. (E,J) hs-ceh-51 embryos accumulate ectopic coelomocytes. (K-M) Bar charts summarizing the hs-ceh-51 data.