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. 2009 Jul 15;136(16):2735–2746. doi: 10.1242/dev.038307

Fig. 6.

Fig. 6.

ceh-51 mutants and tbx-35 mutants raised at 15°C arrest as larvae with pharynx structural defects. Pharynxes were visualized by DIC optics (A,D,G), ceh-22::GFP expression (B,E,H) (Okkema and Fire, 1994) or phalloidin staining (C,F,I) (Franks et al., 2006). In the DIC panels, the lumen (arrows), grinder (gr), metacorpus (mc) and terminal bulb (tb) are indicated and the pharynx is outlined (dashed line). (A-C) Wild-type pharynx. (D-F) ceh-51(tm2123) pharynxes show lumen irregularities and an indistinct metacorpus (D). Protrusions accumulate GFP outside the pharynx, suggesting a defect in pharynx integrity (E). In F, phalloidin staining shows actin filament accumulations (large arrowheads), lumen abnormalities (small arrowheads) and an abnormal terminal bulb (arrow). (G-I) tbx-35(tm1789) raised at 15°C has a normal lumen but abnormal grinder (G). ceh-22::GFP expression (H) shows absence of expression of ceh-22::GFP in part of the posterior pharynx (arrowhead); contralateral expression in this region is likely to be in an MS-derived m7 muscle (Okkema and Fire, 1994; Sulston et al., 1983). In I, phalloidin staining shows some actin accumulations (arrowhead) and an abnormal terminal bulb (arrow).