ceh-51 mutants and tbx-35 mutants raised at 15°C
arrest as larvae with pharynx structural defects. Pharynxes were
visualized by DIC optics (A,D,G), ceh-22::GFP expression (B,E,H)
(Okkema and Fire, 1994) or
phalloidin staining (C,F,I) (Franks et
al., 2006). In the DIC panels, the lumen (arrows), grinder (gr),
metacorpus (mc) and terminal bulb (tb) are indicated and the pharynx is
outlined (dashed line). (A-C) Wild-type pharynx. (D-F)
ceh-51(tm2123) pharynxes show lumen irregularities and an indistinct
metacorpus (D). Protrusions accumulate GFP outside the pharynx, suggesting a
defect in pharynx integrity (E). In F, phalloidin staining shows actin
filament accumulations (large arrowheads), lumen abnormalities (small
arrowheads) and an abnormal terminal bulb (arrow). (G-I)
tbx-35(tm1789) raised at 15°C has a normal lumen but abnormal
grinder (G). ceh-22::GFP expression (H) shows absence of expression
of ceh-22::GFP in part of the posterior pharynx (arrowhead);
contralateral expression in this region is likely to be in an MS-derived m7
muscle (Okkema and Fire, 1994;
Sulston et al., 1983). In I,
phalloidin staining shows some actin accumulations (arrowhead) and an abnormal
terminal bulb (arrow).