Gpr48 regulates bone formation postnatally. (A-D) H&E
staining of tibia of wild-type (A,C) (10×) and
Gpr48-/- (B,D) (10×) mice at P18. There is much less
trabecular bone in Gpr48-/- than wild-type mice, and the
cortical bone is much thinner in the mutant tibia (arrows). (E-H)
Transverse (E,F) and frontal (G,H) microCT sections of distal and midfemoral
diaphyses of 1-month-old wild-type and Gpr48-/- mice.
(I,J) von Kossa staining of L3 vertebral bodies from 1-month-old
wild-type and Gpr48-/- mice. There is a significant
reduction in bone perimeter, bone area and trabecular width in
Gpr48-/- mice. (K,L) Osteoid synthesis
defect in Gpr48-/- mice demonstrated with Goldner staining
(osteoid is stained red, yellow arrows). Deletion of Gpr48
significantly reduced osteoid formation (compare K with L).
(M,N) Masson-Trichrome staining of osteoid (blue).