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. 2009 Jul 24;136(16):2767–2777. doi: 10.1242/dev.034454

Fig. 6.

Fig. 6.

Effect of overexpressing aPKC-CAAX or NLS-aPKC-CT on the neuronal population born during gastrulation. Xenopus embryos were unilaterally injected at the 2-cell stage with either aPKC-CAAX or NLS-aPKC-CT, BrdU saturated at st.10.5 and immunostained for BrdU and for the neuronal differentiation marker X-MyT1 on sections of tailbud at st.25. (A) Top row shows anti-aPKC staining (green), with nuclei in blue (DAPI), to distinguish between injected and non-injected sides for aPKC-CAAX and NLS-aPKC-CT injections. Bottom row shows adjacent sections co-stained for BrdU (red) and X-MyT1 (green). Neural progenitors are positive for BrdU only and appear red, neurons born after BrdU labelling are co-labelled and appear orange (arrow), and neurons born before st.10.5 (BrdU saturation) appear green. The number of neurons born before st.10.5 (green) is reduced after overexpression of either aPKC construct. The dashed lines in circle delineate the neural tube and the straight dashed lines mark the midline of the neural tube. (B) Quantification of the results from A.