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. 2009 Jul 24;136(16):2767–2777. doi: 10.1242/dev.034454

Fig. 7.

Fig. 7.

Effect of Lgl2 overexpression on primary neurogenesis. Overexpression of Lgl2 (1 ng) had no effect on expression of the primary neuron marker N-tubulin (n=35) (D-F) by comparison with control embryos injected with lacZ mRNA only (0.25 ng, n=32) (A-C). But when Lgl2 was co-expressed with the proneurogenic factor X-ngnr-1 (0.25 ng, n=26) (J-L), ectopic primary neurons were found in a position intermediate between the superficial and deep layers (L, arrow), whereas embryos injected with X-ngnr-1 alone (n=41) (G-I) showed ectopic neurogenesis only in the deep layer of neuroectoderm (I, arrow). Also note the absence of lacZ staining in the superficial layer cells of embryos injected with Lgl2 and X-ngnr-1 (L, arrowhead), as compared with lacZ (C) or X-ngnr-1 (I) injected embryos, indicating the possibility of internalisation of superficial cells due to Lgl2 overexpression. Scale bars: 250 μm.