Table 1.
Injection of the to27d splice-blocking morpholino partially rescues the Ite phenotype
| Allele | Dose of MO (ng) | n | Wild type (%) | Mild collapse (%) | Moderate collapse (%) | Severe/complete collapse (%) |
|---|---|---|---|---|---|---|
| to27d | 0 | 917 | 689 (75.1) | 0 (0) | 2 (0.2) | 226 (24.6) |
| 3 | 313 | 237 (75.7) | 5 (1.6) | 59 (18.8) | 12 (3.8) | |
| 4 | 607 | 443 (73.0) | 21 (3.5) | 59 (9.7) | 84 (13.8) | |
| tg414b | 0 | 284 | 196 (69.0) | 0 (0) | 0 (0) | 88 (31.0) |
| 3 | 156 | 106 (67.9) | 0 (0) | 0 (0) | 50 (32.1) | |
| 4 | 184 | 142 (77.2) | 0 (0) | 0 (0) | 42 (22.8) |
Embryos were scored according to their degree of ear collapse at 6 dpf. Similar results were seen at 5 dpf with a graded response from 1 to 7.5 ng morpholino (MO). percentages were rounded to one decimal place.