Figure 2. 5-azaC induced KIR molecules are functional.

T cells were stimulated with PHA and treated with 5-azaC as in Fig. 1. A. The 5-azaC treated T cells were fractionated into CD4+ and CD8+ cells using magnetic beads, then cultured with immobilized anti-KIR2DL4 or an isotype matched IgG and IFN-γ release measured by ELISA. The light hatched bars represent 5-azaC treated cells and the dark bars untreated cells. B. The untreated T cells were cultured with ⁵¹Cr-labelled autologous monocytes/Mø and the indicated concentrations of anti-KIR-3DL1 or isotype matched control IgG, and ⁵¹Cr release was measured 18 hours later. Results are presented as the mean±SEM of 3 determinations.