Figure 2.

Ca uptake vs pulse width as a function of pulse height for rat liver mitochondria. Pulses were made using 2.4 mM EGTA and 2.7 mM ⁴⁵CaCl2-buffered with HEDTA to a free calcium in the pulse medium of 1μM. The experiment was run in HEPES-buffered 150 mM KCl (pH 7.2) with 5 mM Ksuccinate present with mitochondria at a concentration of 0.5 mg/ml. the pulse heights are as follows: □, 474 +/− 14 nM with ruthenium red; ●, 165 +/− 12 nM; ■, 307+/−7nM; ◆, 408 +/− 8 nM; ▲, 567 +/− 8 nM; ▼;, 719 +/− 2 nM; ○, 877 +/− 9nM. Error bars are 1 standard deviation. (Reprinted from Gunter, T. E., Buntinas, L., Sparagna, G. C., and Gunter, K. K. (1998) The Ca²⁺ Transport Mechanisms of Mitochondria and Ca²⁺ uptake from Physiological Type Ca²⁺ Transients. Biochim. Biophys. Acta 1366, 5–15 with permission from Elsevier Science.)