Figure 3. Knockdown of UNG by RNAi-modified pro-arrest and pro-apoptotic gene expression in prostate cancer cells.

Protein (A and B) and mRNA (C and D) levels of various genes in prostate cancer cells transfected with siUNG or controls. Densitometric analysis of protein or mRNA levels were normalized to corresponding GAPDH level and expressed as percentage of Silentfect control.

(A) The cells were transfected with siUNG (10–200 nM) or siMM (200 nM) for 24 h. Proteins were analyzed by immunoblotting.

(B) Cells were transfected with siUNG (100 nM) for various time periods. Proteins were analyzed by immunoblotting.

(C) Cells were transfected with siUNG (10–200 nM) or siMM (200 nM) for 24 h. Target mRNAs were analyzed by RT-PCR.

(D) Cells were transfected with siUNG (100 nM) for various time periods. Target mRNAs were analyzed by RT-PCR.