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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Mol Cancer Res. 2009 Aug 11;7(8):1285–1293. doi: 10.1158/1541-7786.MCR-08-0508

Figure 4. Effects of siUNG alone or in combination with H2O2 or doxorubicin on apoptosis in prostate cancer cells.

Figure 4

The cells were transfected with siUNG or siMM at various concentrations for 12 h (A, LNCaP) or 24 h (B, DU145; C, PC3). In combination treatment with H2O2, the cells were transfected with 50 nM siUNG or siMM for 12 h (D, LNCaP) or 24 h (E, DU145; F, PC3) and then exposed to H2O2 for an additional 12 h (D) or 24 h (E and F). In combination treatment with doxorubicin, the cells were transfected with 50 nM siUNG or siMM for 12 h (G, LNCaP) or 24 h (H, DU145; I, PC3) and then exposed to doxorubicin for an additional 12 h (G) or 36 h (H and I). Cells that stained positive for annexin V-FITC (early apoptosis) and positive for FITC and propidium iodide (late apoptosis) were counted. Relative levels of apoptotic indices were expressed as percentage of Silentfect control. (D–I) Filled circles, Silentfect control; filled triangles, siUNG 50 nM; empty circles, siMM 50 nM.