(A) RT-PCR analyses of undifferentiated, differentiated, and Ang1 stimulated PC12 cells for the presence of Tie2, Ang1, NGF, TrkA and β-actin. PC12 cells express Ang1 and TrkA, but not Tie2 or NGF. (B) Western blotting completed on whole cell lysate harvested from undifferentiated, differentiated, and Ang1 stimulated PC12 cells demonstrates the presence of β1 integrin. The positive control (+) in A represents mouse universal reference total RNA and in B is brain whole cell lysate; the negative control in A is water.