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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Neurosci Res. 2009 Apr 18;64(4):348–354. doi: 10.1016/j.neures.2009.04.007

Figure 4. Ang1 and NGF mediate neurite outgrowth using different signaling pathways.

Figure 4

Western blot analyses and densitometry quantification of downstream β1 integrin signaling partners. NGF and not Ang1 induces a ~3.1 and ~2 fold increase in MAPK (A) and JNK phosphorylation (B) respectively; the addition of β1 integrin function blocking antibodies attenuates NGF-induced JNK activation only. Ang1, but not NGF, induced a ~2.6 fold increase in FAK phosphorylation at tyrosine residue 397 (C). Addition of β1 integrin-specific function-blocking antibodies either in the presence of Ang1 or NGF, or alone also lead to ~3-4 fold increase in FAK (Tyr397)-activation. Ang1 elicited small statistically significant increases in activation of Akt (D) which were decreased following addition of β1 integrin antibodies. *p<0.05.