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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Neurosci Res. 2009 Apr 18;64(4):348–354. doi: 10.1016/j.neures.2009.04.007

Figure 5. SiRNA targeting β1 integrin or functional inhibition of FAK or PI3K signaling prevents Ang1 mediated neurite outgrowth of PC12 cells.

Figure 5

PC12 cells pretreated with NGF for two days were transfected either with Predesigned Silencer® SiRNA against β1 integrin or a negative control random sequence and western blot analyses were completed to confirm decreased β1 integrinexpression at 2, 5, and 7 days post SiRNA transfection (A). Ang1- and NGF-mediated neurite outgrowth assays were then performed in the presence and absence of either the PI3K inhibitor, Wortmannin, the FAK inhibitor PF573288 or following transfection with the SiRNA targeting β1 integrin or a negative control random sequence (B). After two days of stimulation, Ang1-mediated neurite outgrowth was reduced to no sera levels in the presence of Wortmannin, PF573288, and β1 integrin SiRNA (B). NGF-mediated neurite outgrowth was only reduced in the presence of Wortmannin. *p<0.01 compared to no sera controls.