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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: J Biol Inorg Chem. 2009 Apr 7;14(6):873–881. doi: 10.1007/s00775-009-0499-3

Figure 4. Specificity of trPe-POL binding and cross-linking.

Figure 4

(a) Interaction of trPe-POL with the Pol DNA duplex or the scrambled DNA duplex. The platinated N4-(aminopentyl)C-TFO was incubated with Pol DNA duplex or scrambled duplex at 4 °C and the reaction mixtures were run on a 20% native polyacrylamide gel. Lane 1: single stranded 17-mer, lane 2: target duplex, lane 3: 1 μM target duplex plus 2 μM POL, lane 4: 1 μM scrambled duplex plus 2 μM POL, lane 5: 1 μM target duplex plus 10 μM trPe-POL, lanes 6-8: 1 μM target duplex plus 2, 4, or 10 μM trPe-POL (respectively). (b) Cross-linking of trPe-POL with the Pol DNA duplex or the scrambled DNA duplex. The platinated N4-(aminopentyl)C-TFO was incubated with Pol DNA duplex or scrambled duplex at 4 °C and the reaction mixtures were run on a 20% denaturing polyacrylamide gel. Lane 1: 1 μM target duplex plus 2 μM POL, lane 2: 1 μM target duplex plus 10 μM trPe-POL, lanes 3-5: 1 μM scrambled duplex plus 4, 10 or 20 μM trPe-POL (respectively), lanes 6-8: 1 μM target duplex plus 4, 10 or 20 μM trPe-POL (respectively) and 200 μM POL.