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. Author manuscript; available in PMC: 2010 May 15.
Published in final edited form as: Mol Cell. 2009 May 15;34(3):333–343. doi: 10.1016/j.molcel.2009.03.012

Figure 2. Systematic variation of bulge position indicates that at least three positions within the BS-U2 duplex are competent for first step catalysis.

Figure 2

(A) Schematic of reporter and U2 constructs used in (B).

(B) Primer extension with a 3′ exon primer indicates that −1, 0 and +1 BS bulge positions allow splicing catalysis. In this and subsequent figures, a band resulting from degradation of pre-mRNA is marked by an asterisk.