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. Author manuscript; available in PMC: 2010 May 15.
Published in final edited form as: Mol Cell. 2009 May 15;34(3):333–343. doi: 10.1016/j.molcel.2009.03.012

Figure 5. Grossly substituted BS-U2 duplexes predominantly impair spliceosome assembly: inappropriately bulged nucleotides predominantly impair splicing catalysis.

Figure 5

(A) Schematic of the BS-U2 duplex used in this figure, with the positions of bulged adenosines indicated.

(B) Primer extension using a 3′ exon primer on total RNA from strains carrying reporters with adenosines bulged from the indicated positions in a grossly substituted BS-U2 duplex and either wt or mutant Prp5.

(C) Graph indicating the fold change in branching efficiency [calculated as (M+LI)/(P+M+LI)] at the −1 and 0 bulge positions as a result of Prp5 mutation.

(D, E) As (B, C), but comparing wt and mutant Prp16.