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. 2009 Sep 8;7(9):e1000188. doi: 10.1371/journal.pbio.1000188

Figure 5. PHO85 regulates G1 transcription via WHI5.

Figure 5

(A) The G1 delay phenotype associated with a cln3Δ pho85Δ strain is dependent on WHI5. Wt (BY263), cln3Δ (BY653), pho85Δ (BY391), cln3Δ pho85Δ (BY4291), and cln3Δ pho85Δ whi5Δ (BY4292) strains were spotted in serial 10-fold dilutions on rich media (YPED) and incubated for 24 h at 30°C. DNA content of exponentially growing cultures was determined by FACS analysis. Liquid growth assays were also performed for these strains and growth rate is reported relative to wt as shown in the bar graph. Graphical representations of growth rates are shown above the bar graph as line plots, where the upper red line represents the growth of WT and the black line shows the growth of each mutant. (B) A cln3Δ pho85Δ strain exhibits defects in SCB-driven gene expression. Wt (BY4302), cln3Δ (BY4303), pho85Δ (BY4304), cln3Δ pho85Δ (BY4305), cln3Δ pho85Δ whi5Δ (BY4306), pho85Δwhi5Δ (BY4308), and cln3Δwhi5Δ (BY4307) strains harboring an integrated SCB-HIS3 reporter were spotted in serial 10-fold dilutions on histidine-containing medium or media lacking histidine and supplemented with 10 or 30 mM 3-AT. Plates were incubated at 30°C for 48 h. We note that the synthetic growth defect of a cln3 pho85 mutant is most pronounced on rich medium (A), and is not as evident when strains are grown on minimal medium.