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. 2009 Sep 8;7(9):e1000188. doi: 10.1371/journal.pbio.1000188

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Huang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) cln3Δ (BY4290), cln3Δ rpd3Δ (BY4297), cln3Δ hos3Δ (BY4296), and cln3Δ rpd3Δ hos3Δ (BY4298) strains harboring a methionine-repressible WHI5 construct (pBA1975) or vector control (pBA228v) were spotted in serial 10-fold dilutions on medium lacking methionine. In a similar experiment, pho85Δ (BY391), pho85Δ rpd3Δ (BY4300), pho85Δ hos3Δ (BY4299), and pho85Δ rpd3Δ hos3Δ (BY4301) strains bearing a galactose-inducible WHI5 plasmid (pBA1973) or appropriate vector control (pEG-H) were spotted in serial 10-fold dilutions on galactose-containing medium. Plates were incubated at 30°C for 48 h. (B) Deletion of HOS3 partially restores growth of a cln3Δ pho85Δ strain. The indicated strains (BY263; BY4291, BY4292, BY4455–4461) were spotted in serial 10-fold dilutions on rich medium (YPED) and incubated at 30°C for 48 h. (C) Deletion of RPD3 and HOS3 partially restore viability of a cln3Δ bck2Δ strain. The indicated strains (BY4741; BY2948, BY4462–4468) were spotted in serial 10-fold dilutions on glucose-containing medium (YPED) to repress CLN3 expression. Strains were also spotted on medium containing galactose as a control. Plates were incubated at 30°C for 72 h.