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. 2009 Sep 8;7(9):e1000188. doi: 10.1371/journal.pbio.1000188

Figure 10. Repression of gene expression by Whi5 is dependent on HOS3 and RPD3.

Figure 10

The growth defects of cln3Δ and pho85Δ strains can be rescued by removing RPD3 and HOS3 in SCB-driven gene expression. Wt (BY4302), cln3Δ (BY4303), pho85Δ (BY4304), cln3Δ rpd3Δ (BY4297), cln3Δ hos3Δ (BY4296), cln3Δrpd3Δhos3Δ (BY4298), pho85Δrpd3Δ (BY4300), pho85Δhos3Δ (BY4299), and pho85Δrpd3Δhos3Δ (BY4301) strains harboring an integrated SCB-HIS3 reporter were spotted in serial 10-fold dilutions on histidine-containing medium or media lacking histidine and supplemented with 10 or 30 mM 3-AT. Plates were incubated at 30°C for 48 h.