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. 2009 Sep 8;7(9):e1000188. doi: 10.1371/journal.pbio.1000188

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Huang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Pho85 and Cdc28 activity inhibits interaction between Whi5 and Rpd3. PCL9^FLAG (pBA1974), CLN2^FLAG (pBA2247), CLN3^FLAG (pBA2248), or a vector control (pMT3164) were introduced into a strain harboring RPD3^TAP at the chromosomal locus (BY4315) and a WHI5^GST plasmid (pBA1973). Cyclin expression was confirmed by immunoblot using anti-FLAG antibodies. Lysates were incubated with glutathione sepharose beads. Whi5^GST-Rpd3^TAP interactions were detected by immunoblot using α-GST and α-PAP antibodies, (B) Pho85 and Cdc28 activity inhibits interaction of Whi5 and Hos3. Experiments were conducted as described in (A) but using a strain bearing HOS3^TAP at the chromosomal locus.