Figure 6. Expression analysis of genes associated with male recombination phenotypes.

(A) Mouse male meiotic cells were purified by FACS from testes. A typical profile is shown with the purified cell fractions circled in red. All meiotic cells were also purified and used as reference sample (circled in blue). (B) Expression patterns for genes associated with male recombination phenotypes. All genes display a strong induction from the onset of meiosis. Relative expression (ΔΔCt) was calculated using mouse β-actin as the endogenous control and the entire meiotic cells as the reference. Error bars indicate ± s.e.m. Sp: Spermatogonia, L/Z: Leptotene/Zygotene, Pa: Pachytene, Di: Diplotene, SS/RS: Secondary spermatocytes and round spermatids.