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. 2009 Sep 1;4(9):e6879. doi: 10.1371/journal.pone.0006879

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Räsänen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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UT-SCC colony formation was studied with soft-agarose assay. All UT-SCC cells formed colonies in soft agarose, recurrent SCC (B and D) twice as many as primary SCC cells (A and C) (P<0.05). Normal fibroblasts increased the number of colonies of primary carcinomas cells and this was further augmented with CAF cells (P<0.05) (A and C). Recurrent SCC cell colony formation was inhibit with normal fibroblasts (P<0.05 in FB-74 compared to control) and restored to control level by CAFs (B and C). Columns: mean; error bars; SEM.