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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Mol Cancer Ther. 2009 Jan;8(1):45–54. doi: 10.1158/1535-7163.MCT-08-0662

Figure 2. The effects of PD-321852, Gem or Gem + PD-321852 on checkpoint protein levels.

Figure 2

Basal levels of Cdc25A, Chk1, Chk2 and Rad51 in pancreatic cancer cell lines (A). Image shown is representative of 2 independent experiments. In (B), cells treated with either Gem alone (100 nM for MiaPaCa and BxPC3, 300 nM for M-Panc96 and 1 μM for Panc1), 0.3 μM PD-321852 or Gem + PD-321852 for 24 h were assayed for Cdc25A, Chk1 and Chk2 protein levels. Breaks indicate non-contiguous lanes from a single membrane. Images shown are representative of 3-5 independent experiments. For quantitation of Chk1 depletion in cells treated with Gem + PD-321852 (C), autoradiograms were scanned for densitometric analysis and normalized first to actin loading control, then to the effects of Gem alone. Data are given as the mean ± SEM (n=3-5), *p<0.05 or **p<0.01, one-way ANOVA. In (D), MiaPaCa2 and BxPC3 cells were treated with Gem + 0.3 μM PD-321852 for 24 h, with no other addition, or with 25 μg/ml LLnL added for the final 3 h of drug treatment. Images shown are representative of two independent experiments.