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. 2009 Aug 31;4(8):e6858. doi: 10.1371/journal.pone.0006858

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Kanerva et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immunoblot from COS-7 cells transfected with plasmids carrying FLAG-tagged AZIN1, AZIN2, or ODC gene or an empty FLAG-vector (Ctrl). An Odyssey Infrared Imager was used for simultaneous two-color detection of rabbit anti-AZIN2 (antiserum 3) and mouse anti-FLAG antibodies. The lowest panel represents merged blots, yellow indicating co-localization of anti-AZIN2 and anti-FLAG. (B) Confocal laser scanning microscopy of COS-7 cells expressing one of the following genes: AZIN2-FLAG, AZIN1-FLAG, or ODC-FLAG. The cells were stained with either AZIN2 antiserum 2 or 3 together with mouse anti-FLAG, followed by donkey anti-rabbit Alexa Fluor-555 and donkey anti-mouse Alexa Fluor-488. AZIN2-FLAG-transfected cells were also stained with preimmune sera of the corresponding rabbits. In the merged images, yellow indicates the co-localization. Bars represent 10 µm.