Table 1.
Effect of Asc on HNE metabolism and excretion in THP-1 cells
| Effect of Asc on HNE conjugate formation expressed as the ratio of peak areas a, |
p-value of effect of Asc c |
|
|---|---|---|
| HNE metabolite | AAsc-adeq, avg /AAsc-def, avg | |
| GSH-HNAL | 1.16 | 0.014 |
| GSH-HNE | 1.12 | 0.11 |
| GSH-DHN | 1.16 | 0.086 |
| GlyCys-HNAL | 1.11 | 0.33 |
| GlyCys-HNE | 1.11 | 0.20 |
| GlyCys-DHN | 1.06 | 0.61 |
| Effect of Asc on HNE conjugate distribution between medium and lysate expressed as the ratio b, |
||
| RAsc-adeq, avg /RAsc-def, avg | ||
| GSH-HNAL | 1.25 | 0.012 |
| GSH-HNE | 1.04 | 0.54 |
| GSH-DHN | 1.21 | 0.013 |
| GlyCys-HNAL | 0.95 | 0.40 |
| GlyCys-HNE | 1.16 | 0.048 |
| GlyCys-DHN | 1.16 | 0.040 |
AAsc-adeq = Peak area recorded for metabolite x in medium + Peak area recorded for metabolite x in lysate of Asc-pretreated THP-1 cells. AAsc-def = Peak area recorded for metabolite x in medium + Peak area recorded for metabolite x in lysate of THP-1 cells that were not pre-treated with Asc. The average (avg) of three measurements was determined to calculate the ratio AAsc-adeq, avg /AAsc-def, avg (n = 3). For instance, a ratio of 1.16 indicates a 16% increase of HNE conjugate formation resulting from Asc-pretreatment.
RAsc-adeq = AAsc-adeq, medium /AAsc-adeq, lysate and RAsc-def = AAsc-def, medium /AAsc-def, lysate. For instance, a ratio RAsc-adeq, avg /RAsc-def, avg of 1.25 indicates a 25% increase of metabolite amount found in the medium relative to the lysate amount, resulting from Asc-pretreatment.
The null hypothesis was AAsc-adeq = AAsc-def or RAsc-adeq = RAsc-def (n = 3).