Table I.
SSLP markers used for genetic mapping.
| Allele Sizeb (bp) | |||||
|---|---|---|---|---|---|
| Marker | Chr. 17 Locusa (bp) | Primers (5′-3′) | C57BL/6 | MA/My | C57L |
| 17Uva12 | 34547552-34547799 | ACCAGCAAGCCCTATGTCAC TATGTCTACTGGTCAGGTTG |
248 | 250 | 245 |
| 17Uva18 | 34707366-34707702 | GTTCAGGGATACGGTGCTCC CCTTCTCTGATAAGACGGTC |
337 | ND | ND |
| 17Uva19 | 35099356-35099606 | GCGTTGTGACCCCTGGAGGC GAATGCCTGTCTCAGCACGG |
251 | ND | ND |
| 17Uva20 | 35143895-35144160 | GGGTGTTGGATGCTCCTGGA TACTGCCGAACCACTTCTCC |
266 | 271 | 266 |
| 17Uva15 | 35341805-35342016 | AGACAATGGCTAACAGAGGC TTCTATACAACCTGCCTGGA |
212 | 225 | 208 |
| 17Uva01 | 35375912-35376112 | GCAGCACTCTGTCTCTTTCC ATAGTAAGTCTCTGTCTCTC |
198 | 182 | 198 |
| 17Uva16 | 35406718-35406802 | CCTCCCATATTGGCTCTTCC GCAGTTGGACCTTTGACAGA |
85 | 126 | 81 |
| 17Uva17 | 35424300-35424711 | AGCGACTGAATATGGGTGAC GCAGGACTAACTGGATTGTC |
412 | 427 | 414 |
| 17Uva03 | 35451303-35451610 | TGCATGGGAAATACTCGTCC GGAGCTGACTCATCTCATTG |
308 | 291 | 311 |
| 17Uva14 | 36458241-36458469 | CCAAGGGCACTGTATTCCTG GACAAGTACCAAGTGCTCCT |
229 | 189 | 199 |
| 17Uva06 | 38389926-38390222 | AGGTGTTTATCAGCTGTAGG CAAGCCAACATTCAACATCA |
297 | 304 | 294 |
| 17Uva09 | 43168363-43168537 | AGCCCTGTCTCAAGTCAACG TCTGTGCCATTGACGTTAGC |
175 | 179 | 155 |
a
Chromosome locations for forward primers are based on C57BL/6 genomic sequence (Build 37, Ensembl Release 52).
b
Allele sizes based on electrophoretic mobility in POP7-filled capillaries run on a Genetic Analzyer 3130xl. 17UVA18 and 17UVA19 allele differences are based on electrophoresis in 4.0% agarose gel (data not shown) but exact sizes are not determined (ND).