Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1979 Apr;9(4):498–502. doi: 10.1128/jcm.9.4.498-502.1979

Immunoglobulin G- and M-specific enzyme-linked immunosorbent assay for detection of dengue antibodies.

D Dittmar, T J Cleary, A Castro
PMCID: PMC273062  PMID: 379040

Abstract

An enzyme-linked immunosorbent assay for the detection of antibodies to dengue virus is described. This method correlates well with a hemagglutination inhibition technique. The enzyme-linked immunosorbent assay can also be specific for human immunoglobulin M antibodies when a mu-chain-specific antiglobulin-enzyme conjugate and fractionated serum are employed. By using this technique, dengue immunoglobulin M antibodies were demonstrated in an infant suspected of having a recent dengue infection.

Full text

PDF
500

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brandt W. E., Cardiff R. D., Russell P. K. Dengue virions and antigens in brain and serum of infected mice. J Virol. 1970 Oct;6(4):500–506. doi: 10.1128/jvi.6.4.500-506.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Castellano G. A., Hazzard G. T., Madden D. L., Sever J. L. Comparison of the enzyme-linked immunosorbent assay and the indirect hemagglutination test for detection of antibody to cytomegalovirus. J Infect Dis. 1977 Oct;136 (Suppl):S337–S340. doi: 10.1093/infdis/136.supplement_2.s337. [DOI] [PubMed] [Google Scholar]
  3. Edelman R., Nisalak A., Pariyanonda A., Udomsakdi S., Johnsen D. O. Immunoglobulin response and viremia in dengue-vaccinated gibbons repeatedly challenged with Japanese encephalitis virus. Am J Epidemiol. 1973 Mar;97(3):208–218. doi: 10.1093/oxfordjournals.aje.a121501. [DOI] [PubMed] [Google Scholar]
  4. Ehrenkranz N. J., Ventura A. K., Cuadrado R. R., Pond W. L., Porter J. E. Pandemic dengue in Caribbean countries and the southern United States--past, present and potential problems. N Engl J Med. 1971 Dec 23;285(26):1460–1469. doi: 10.1056/NEJM197112232852606. [DOI] [PubMed] [Google Scholar]
  5. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry. 1971 Sep;8(9):871–874. doi: 10.1016/0019-2791(71)90454-x. [DOI] [PubMed] [Google Scholar]
  6. Fenner F., Pereira H. G., Porterfield J. S., Joklik W. K., Downie A. W. Family and generic names for viruses approved by the International Committee on Taxonomy of Viruses, June 1974. Intervirology. 1974;3(3):193–198. doi: 10.1159/000149755. [DOI] [PubMed] [Google Scholar]
  7. Fujita N., Tamura M., Hotta S. Immuno-biological studies on dengue. I. Immunogenic effects of partially purified dengue-1 virus and some of its components. Kobe J Med Sci. 1976 Sep;22(3):211–228. [PubMed] [Google Scholar]
  8. Leinikki P., Pässilä S. Solid phase antibody assay by means of enzyme conjugated to anti-immunoglobulin. J Clin Pathol. 1976 Dec;29(12):1116–1120. doi: 10.1136/jcp.29.12.1116. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McCracken A. W., Newman J. T. The current status of the laboratory diagnosis of viral diseases of man. CRC Crit Rev Clin Lab Sci. 1975 Jan;5(3):331–363. doi: 10.3109/10408367509107047. [DOI] [PubMed] [Google Scholar]
  10. Scott R. M., McCown J. M., Russell P. K. Human immunoglobulin specificity after group B arbovirus infections. Infect Immun. 1972 Sep;6(3):277–281. doi: 10.1128/iai.6.3.277-281.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Trent D. W. Antigenic characterization of flavivirus structural proteins separated by isoelectric focusing. J Virol. 1977 Jun;22(3):608–618. doi: 10.1128/jvi.22.3.608-618.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Vathanophas K., Hammon W. M., Atchison R. W., Sather G. E. Attempted type specific diagnosis of dengue virus infection by the indirect fluorescent antibody method directed at differentiating IgM and IgG responses. Proc Soc Exp Biol Med. 1973 Feb;142(2):697–702. doi: 10.3181/00379727-142-37097. [DOI] [PubMed] [Google Scholar]
  13. Ventura A. K., Ehrenkranz N. J., Rosenthal D. Placental passage of antibodies to Dengue virus in persons living in a region of hyperendemic Dengue virus infection. J Infect Dis. 1975 May;131 (Suppl):S62–S68. doi: 10.1093/infdis/131.supplement.s62. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES