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Compound 9 (ALW-II-49–7) inhibits EphB2 tyrosine kinase activity. The U87 glioblastoma cells were either left untreated or treated for 1 hr with indicated concentrations of ALW-II-49–7 in the presence of ephrinB1. Total lysates were subject to immunoprecipitation with an anti-EphB2 antibody, and immunoprecipitated EphB2 was analyzed by western blot with an anti-phosphotyrosine antibody (top). The blot was reprobed with an anti-EphB2 antibody to show equal loading of proteins (bottom).
