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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Dev Biol. 2009 May 27;332(1):166–176. doi: 10.1016/j.ydbio.2009.05.566

Fig. 8. Jag1-Notch signaling regulates Jag1 induction.

Fig. 8

(A) CE explants were cultured in the presence of FGF for 4 days in the presence or absence of anti- Jag1 antibody and analyzed for expression of Jag1. Immunoblotting demonstrated a marked reduction of Jag1 protein in the presence of anti-Jag1 antibody. (B) Quantification of Jag1expression by densitometric scanning of replicate immunoblots (n=3), demonstrated approximately a 70% decrease +/− 10.9 (s.e.m) (p=0.0238). (C) CE explants were cultured in the presence of FGF for 4 days in the presence or absence of the gamma secretase inhibitors, DAPT (50μM) and L-685,458 (20μM). Inhibition of Notch signaling with these pharmacological inhibitors confirmed that Notch signaling regulates induction of Jag1.