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. Author manuscript; available in PMC: 2010 Mar 1.

Published in final edited form as: Int J Med Microbiol. 2008 Sep 30;299(3):187–201. doi: 10.1016/j.ijmm.2008.07.002

Fig. 2 — Immunofluorescence analysis of inclusion size and shape in HEp-2 (Panels A–C), U-87 MG (Panels D–F), and CHME-5 (Panels G–I) cells infected with C. pneumoniae isolates Phi-1 (Group I[A–I], left) and Tor-1 (Group II[A–I], right) at 24 h p.i. (Panels A, D, G), 48 h p.i. (Panels B, E, H), and 72 h p.i. (Panels C, F, I). Cells were infected on glass cover slips at MOI 0.5 for HEp-2 cells and MOI 1 for U-87 MG and CHME-5 cells. Infected cells were stained with the Pathfinder™ mAb, which targets the chlamydial LPS. Infected cells were visualized using epifluorescence on a Nikon E600 microscope. The scale bar in Panel A in each group represents 10 µm, and all photomicrographs were taken at the same magnification in both large groups.

Fig. 2 — Immunofluorescence analysis of inclusion size and shape in HEp-2 (Panels A–C), U-87 MG (Panels D–F), and CHME-5 (Panels G–I) cells infected with C. pneumoniae isolates Phi-1 (Group I[A–I], left) and Tor-1 (Group II[A–I], right) at 24 h p.i. (Panels A, D, G), 48 h p.i. (Panels B, E, H), and 72 h p.i. (Panels C, F, I). Cells were infected on glass cover slips at MOI 0.5 for HEp-2 cells and MOI 1 for U-87 MG and CHME-5 cells. Infected cells were stained with the Pathfinder™ mAb, which targets the chlamydial LPS. Infected cells were visualized using epifluorescence on a Nikon E600 microscope. The scale bar in Panel A in each group represents 10 µm, and all photomicrographs were taken at the same magnification in both large groups.