Table 2.
Chlamydial genes targeted and primers used for PCR and/or RT-PCR assays.
| C. pneumoniaea | Primers Employed | Product size |
|---|---|---|
| ompA (Cpn0695) | 5'-tattatccgccgcatttg-3' | 535 bp |
| 5'-gaggtgtctgtgtaaagttc-3' | ||
| Cpn1046 | 5'-gaaggttgctccactgatatg-3' | 729 bp |
| 5'-agtgagttctaccagttcatc-3' | ||
| C. trachomatis | ||
| plasmid ORF4 (pCt02) | 5’-ttccccttgtaattcgttgc-3’ | 201 bp |
| 5’-tagtaactgccacttcatca-3 | ||
| C. caviaeb | ||
| incA | 5'-gctgctgctgtaggggctaaaacggc-3' | 849 bp |
| 5'-ctctgcaccatcctgaaccataacacc-3' | ||
| C. psittacic | ||
| Cps0300 | 5'-catcgagtcgctatctct-3' | 871 bp |
| 5'-ccagttcgtccacctc-3' |
a
Gene sequence for each C. pneumoniae and C. trachomatis gene or transcript examined available at www.stdgen.lanl.gov; see also Stephens et al. (1998) and Read et al. (2000).
b
Full genome sequence, including the sequence for the incA gene, available at GenBank accession number AE015925.
c
Gene sequence generously supplied to A.P.H. by G.S.A. Myers, pers. comm.