Fig. 4.

Kinetics of inflammatory cell numbers and percentage of cellular subsets during infection with Mycobacterium ulcerans strains from Mexico and Benin. The total number of inflammatory cells (a) and the percentage of CD4 (b), CD8 (c) and macrophages CD11b⁺ (d) was determined by immunohistochemistry and automated morphometry in frozen sections from animals infected with strain 5114 from Mexico (black bars) and isolate 1441 from Benin (white bars). Three different randomly chosen areas at 400× magnification from the subcutaneous tissue of four different mice per time-point were studied. Data are expressed as means and standard deviation; asterisks represent statistical significance (P < 0·005).