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. 2009 May 19;94(8):3065–3071. doi: 10.1210/jc.2009-0542

Figure 1.

Figure 1

NGF increases mRNA expression in hGCs. A, Ethidium bromide-stained gel showing the presence of VEGF mRNA transcripts encoding the VEGF isoforms 121 and 165 in cultured hGCs, as measured by RT-PCR. MM, Molecular markers; (+), positive control (ovarian cancer); (−), negative control (PCR mix without cDNA); C, hGCs cultured under basal conditions; N, hGCs cultured for 8 h in the presence of 50 ng/ml NGF. This gel is representative of seven independent experiments. B, Effect of NGF on VEGF121 mRNA levels measured by semiquantitative PCR after different times of treatment (2, 4, 8, and 18 h). Each column represents the mean ± sem of seven independent experiments, each assayed in duplicate. C, Effect of NGF on VEGF165 mRNA levels. *, P < 0.05; **, P < 0.01 vs. untreated controls. AU, Arbitrary units.