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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2009 Apr 2;29(6):921–928. doi: 10.1161/ATVBAHA.109.187229

Figure 1. Effects of depletion Brg1 or Brm on expression of endogenous smooth muscle-specific genes.

Figure 1

Mouse aortic smooth muscle cells were transfected with pre-designed Dharmacon siRNA smart pools targeting Brg1 or Brm, as well as a control siRNA, at final concentration of 50nM. 36 h after transfection, mRNA was harvested and the levels of Brg1, Brm and smooth muscle marker genes were measured by quantitative real time RT-PCR as indicated in each panel. Data presented are the mean±SEM of 6 samples from 2 independent experiments and expression levels were normalized to an hprt internal control and are expressed relative to levels in siRNA control samples (set to 1). * Indicates statistical significance as determined by a student T test (P<0.05).