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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Psychiatr Genet. 2009 Apr;19(2):72–82. doi: 10.1097/YPG.0b013e3283208091

Fig. 1. Assessment of the quality of DNA generated by different extraction methods.

Fig. 1

A. A volume of 20 μl of prepared DNA was run on 1% agarose gel, either uncut (lanes 3, 5, 7, 9) or after digestion with EcoRI (lanes 4, 6, 8, 10). Lane 1: commercial 1kb DNA ladder; lanes 3-4: commercially purchased DNA. lanes 5-6: DNA prepared by BuccalAmp kit; lanes 7-8: DNA prepared by the rapid method (RM); lanes 9-10; DNA prepared by the modified rapid method (RMS) B. samples of DNA purified by RM or by RMS with different buffer conditions were run on 1% agarose gel or digested with EcoRI and run on agarose gel. Lane 1: 500bp DNA ladder; lanes 2-7: undigested DNA; lanes 8-13: DNA digested with EcoRI. DNA was visible for commercial DNA (lanes 2, 8), and for RMS DNA prepared at buffer pH of 5.4 (lanes 3, 9), 7.6 (lanes 4, 10), and 10 (lanes 5, 11). Preparations from the RMS method adjusted by NaOH (lanes 6, 7, 12, 13) were also run.