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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Psychiatr Genet. 2009 Apr;19(2):72–82. doi: 10.1097/YPG.0b013e3283208091

Fig. 2. Spectral analysis of DNA extracted by different methods.

Fig. 2

DNA was extracted from buccal swabs by ten different methods as described in the text. A spectrum for 100μl of each was generated at 220nm to 300 nm with a Beckman DU-70 spectrophotometer. On each spectrum, the position of OD260 is indicated with a dashed line. The major peak is indicated with an arrow. Samples analyzed were as follows: A. Commercial DNA sample, 100ng diluted into 100μl. B. Rapid Method (RM), C-H. Modified rapid method (RMS) with different buffer conditions, specifically pH 5.4 (C), pH 7.6 (D), pH 10 (E), 50mM NaOH (F), and 50mM NaOH equilibrated with 1M Tris pH 7.6 (G), Epicentre buffer used with RMS (H). I. The conventional Epicentre BuccalAmp method. J. TKM buffer used with the BuccalAmp `heat and vortex' method.