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. Author manuscript; available in PMC: 2009 Aug 24.
Published in final edited form as: Breast Cancer Res Treat. 2007 Sep 20;110(3):493–505. doi: 10.1007/s10549-007-9748-8

Fig. 1.

Fig. 1

The expression of PTHrP and erbB receptors in a panel of human breast cancer cell lines. (a) Relative PTHrP mRNA levels in the various cell lines. The relative ratios of PTHrP mRNA to GADPH mRNA were expressed as a means ± s.d. of triplicate cultures from a single experiment. This experiment was repeated 3 times with similar results. (b) Cell extracts were prepared from unstimulated cells and quantified by a Bradford assay. Glyocoproteins from 500 μg of protein were precipitated with concanavalin A and analyzed. The immunoblots were probed with the corresponding antibodies. This experiment was repeated twice