Fig. 5.
Inhibition of AREG and EGFR signaling decreases PTHrP expression. (a) MDA, (b) A1, (c) S1 or (d) BC61 cells were treated with 1 μM of PD for 6 h, 10 μg/ml of an AREG neutralizing antibody for 6 (a) or 24 h (b, c), or 10 μg/ml of goat IgG as a control. Messenger RNA was harvested from three independent cultures and analyzed. This experiment was repeated twice. *P < 0.01 vs. basal